邢唷��>� DF��C��欹�� bjbj!! 00CfCf' a"�� 8LD�,�-t�(�:2"2"2"Z-\-\-\-\-\-\-$O0�3�€-P#� �2"P#P#€-��l�-8'8'8'P#��Z-8'P#Z-8'8':(*tf��*��饚鬔u��J$v�* F-�-0�-�*.�3�$H�3�*�*&�3�*L2"L~"68'�",�"p2"2"2"€-€-&02"2"2"�-P#P#P#P#��32"2"2"2"2"2"2"2"2"� �: ANTISERA REQUEST FORM Completed forms should be saved as a PDF and submitted to Comparative Medicine at HYPERLINK "mailto:CompMed@research.usf.edu" CompMed@research.usf.edu for uploading into the ARC system for IACUC consideration. HYPERLINK 1. REQUESTING FACULTY MEMBER. Requesting Faculty Member抯 Name Department Campus Mailbox Phone 2. ALTERNATIVES, LITERATURE SEARCH, PRINCIPLES & PROCEDURES. Indicate by checking, that: A search for alternatives was conducted on ___________________ (date) of PubMed covering the years 20___ - 20___ ( > last 10 years) using the search terms animal, rabbit, research, pain, antiserum, antibody production, alternatives, antigen, inflammation, adjuvant, immunogen, early endpoint, anesthesia, analgesia, exsanguination, polyclonal antibodies, RIBI�, TiterMax�, MPL�, SAF�, and the specific immunogen & adjuvant_______________________________________________________ A search for alternatives was conducted on ___________________ (date) of Web of Science covering the years 20___ - 20___ (> last 10 years) using the search terms animal, rabbit, research, pain, antiserum, antibody production, alternatives, antigen, inflammation, adjuvant, immunogen, early endpoint, anesthesia, analgesia, exsanguination, polyclonal antibodies, RIBI�, TiterMax�, MPL�, SAF�, and the specific immunogen & adjuvant________________________________________________ Non-animal alternatives were considered, less sentient species were considered, and none were found suitable for antiserum production Alternatives to painful procedures were considered, none were found suitable, and methods used in antiserum production will be continuously refined so as to reduce animal discomfort Animal use for antiserum production does not unnecessarily duplicate previous research or existing studies Antiserum production using NZW rabbits by Comparative Medicine in accordance with IACUC Principle XVII is requested without exception, including all administrations, observations, phlebotomies, and treatments made and recorded by Comparative Medicine, and that the requesting investigator will provide the immunizing antigen to the Facility Manager, who will in turn coordinate and supervise the service 3. ANTIGEN PREPARATION. Name and describe the antigen(s) of interest (e.g., IL-1 receptor), and the preparation(s) (e.g., soluble, insoluble or particulate protein, or peptides conjugated to carrier proteins, or carbohydrates, etc.): Antigen(s): Preparation(s): 4. ANIMALS. Indicate the total number of rabbits requested (i.e., 2-4 rabbits/antigen): 5. SPECIAL CONSIDERATIONS. Check only if applicable, that: Complete Freund's Adjuvant (CFA) is required for this request The searches for alternatives above includes the search terms CFA, anesthesia, exsanguination, polyclonal antibodies, adjuvant, pain, abscess, alternative adjuvants, RIBI�, TiterMax�, MPL�, SAF�, and early endpoint The following scientific justification is provided for the use of Complete Freund抯 Adjuvant: __________________________________________________________________________________________________ Requesting Faculty Member抯 Signature Date PAGE 1 CMDC #203.1 Effective 11/15 Page PAGE 1 of NUMPAGES 1 Cdhi�� 庹榷榷ざ日庹庹湗湈~sh]Sh�[CJOJQJhZC�5丆JOJQJhwg5丆JOJQJh�[5丆JOJQJh#?h1y�5丆JOJQJaJh�[CJOJQJh@Q<�jh@Q<�U#h瀓5h�$�0J5丆JOJQJ^J"jh�$�5丆JOJQJU^Jh�$�5丆JOJQJ^Jh1y�5丆JOJQJ^Jh!1h!15丆JOJQJ^J h�[CJh�[h1y�� : ; � � � � � �� FH�kl��勑^勑gd1y�勑^勑gd:�gd1y�$7$8$H$a$gd1y�gd1y� 9 : ; V ^ i t v | � � � � � � � � � � � 鲡厥弓灜瀿瓐灜|瀕aVKaCh1y�OJQJhZC�5丆JOJQJh1y�5丆JOJQJh�[5丆JOJQJh#?h�[5丆J OJQJaJ hNO�CJOJQJ^Jh1y�CJOJQJh1y�CJOJQJ^Jh�4h�[CJOJQJ^JhwgCJOJQJ^J h�$�h�[CJOJQJ^JaJh1y�h�[5丆JOJQJh�[CJOJQJ&jh#?CJOJQJUmHnHuh#?CJOJQJ� � � � � � � & ' > E H O W \ a b d f l m n o p r s t y � � 0;_��%(嵯丘鸺牀尨羌鴦区区h�$�OJQJh��h��OJQJh��>*OJQJh��h��>*OJQJh��OJQJh:_�OJQJh1y�h1y�OJQJh?m�OJQJ"jh:�OJQJUmHnHuh鱎�h1y�CJ OJQJaJ h1y�OJQJh:�OJQJ0(67@DRSUVWXYZ[`jk� # G p � � � � � � � FGH��klm遢蛰蛰蛰蛰烧蓓屙枕屙辶屙く湦瘻敜湳zoh�[5丆JOJQJhZC�5丆JOJQJh#?h1y�CJ OJQJaJ h榙�OJQJh1y�OJQJh1y�h1y�OJQJ"jh:�OJQJUmHnHuh�$�OJQJh��h��>*OJQJh��OJQJh:_�OJQJh:�OJQJh1y�h:�OJQJh憓lOJQJ*�� -yz��02345�')*,-/��gdIT勑^勑gd�-�gd�-�� !$%+-.5wyz��蹶仄径径杽ynynf秄禫nNFNFh�-�OJQJh9"pOJQJh#?h�[5丆J OJQJaJ h?蔼础叠颁顿闯惭翱窜摆袄冲别蹿濒尘苍辞蝉迟词赌��耖彘彘彘遽葆萦陀掠途喊皽挏皠皠耻劙劙剈劙横葆�丑闯颁闯翱闯蚕闯尘贬苍贬耻箩丑!1颁闯翱闯蚕闯鲍丑补诲痴颁闯翱闯蚕闯丑憓濒颁闯翱闯蚕闯丑�$�颁闯翱闯蚕闯丑驰�颁闯翱闯蚕闯丑蔼蚕<�hY�hY�0JmHnHu hY�0Jjh!10JUh��hadVh�%�jh�%�UhIThITCJOJQJaJ./0234567BCDP`��gd:�匋��&`#$��痃沌沌沌沌沌鹪硼古皙硼○橉○墇r瘘chIThITCJOJQJaJhwgOJQJhwghwgCJOJQJaJhwgh9"pCJOJQJaJh9"p5丱JQJh�-�5丱JQJh�$�hY�CJOJQJaJhY�CJOJQJaJh�$�h�$�CJOJQJaJh�$�hZC�CJOJQJaJh:�h:�5丱JQJh@Q<�hY�OJQJh�%�$��gd9"pgd�-�gdZC�gd:��gdIT.:p#?靶/ 班=!癅"癅#愋$惏%�靶�愋^2�� 0@P`p€��2(�� 0@P`p€�� 0@P`p€�� 0@P`p€�� 0@P`p€�� 0@P`p€�� 0@P`p€�8X�V~_HmH nH sH tH 8`�8!1Normal_HmH sH tH <�@<�!1 Heading 1$@&OJQJDA`��D Default Paragraph FontRi��R 0Table Normal�4� l4�a�(k ��( 0No List:B@�:!1 Body TextCJOJQJBP@B!1Body Text 25丆JOJQJ4 @4!1Footer ��!.)@�!.!1Page Number4@24!1Header ��!:>@B:!1Titlea$5丆JOJQJH橜RH!1Balloon TextCJOJQJ^JaJ6U`�a6!1 Hyperlink>*B*ph�B��qB�,apple-converted-spacef矦�f�:� List Paragraph勑dと^勑m$CJOJPJQJ^JaJB'`��B?m�Comment ReferenceCJaJ4@�4?m�Comment Text:��:?m�Comment Text CharLj@��L?m�Comment Subject5乗乵HsHtHF䁖��F?m�Comment Subject Char5\PK!檗�[Content_Types].xml瑧薔�0E鱄鼉�-J湶@%閭菐洽|廊�$韶钵UL襎B� l,�3鳛;鉹�得槣B+$�G]ミ7O侪V墎$�┇最�!)O赹齬虲$駓@摪磔�/瓂H*�橊劥�)戅祶鬟粖譛Db俙}"譹蹕擩讞枻肵^�)I`n蘀�紛p)�杵li筕[]�1M<斷绒彥O蠵擊6r�=瘔抸纆b營g吜u崘S賓b嘱€O缽嗕掘肦罝郢櫉反qu 痝嫎Z岸串o~俸lAp發x妏T0�+[}`j纂鹾絲A�帮儲V�2虵蒳朄鰍瀡分�5\|夻蕼汰Nвle瞂�ds趈cs倭惻7琊嵨f坊赅肉W琊�+唻7爤唁`�陲g�葮稠J�雷j|唫h(�驞-姷咩� dX�﹊J曝�(钼x$(��:隶;渌�!�I_蠺到S1ｗ犍鳢�?E��?勉?ZB为m渼錟/魁煜��?瀪篁�誼Y�'奎鼀5襣&螊/燑鲮蓩�>籊餗丟e鴲艱�眢3Vq%'#q��域娡$�8翚K秊�敉)f檞9:牡�澹 x}r�x墘�渨⒇顁�:\TZaG�*檡8I耲鎎R祈c|X呕�强絀 u3KG駈D1�NIB襰鼆� 眍R曦u楘侹>V�.EL+M2�#'歠嫸i~橵�l硔u8z�篐� �*�鏄�:�(W�鈽� ~J攘T鴈\O*餿HG絸HY垫�}KN吡P�*菥甩眿�T鸭�9/#辐A7聁Z��$*c?��韖U咤n嗚w�N蝴%幓O�穒鑸4=3炯N�績)cbJ u�4峦(Tn酸� 7斒_?異鹠-贈皗U逄鰤B�w�襷消╪湐鞘�"Zx娬J躗氺p;嫇熟劐辿/�<�P;檸,)''K蠶踜5棝騫邛苝N喦8疜軬b耬摨� 鸖撡d洯\17 阷鮝�>ОSR!�枒 3晠K4'+�r蘻Q TT３I辈琉疘vt]K芻猥渤K#趘�5+D�眽厍鄜獱O@%\w槉燺鄋N[跮古9K候崢临q桃g錠炆n R!儁+�篣蕁�;�*&�/H時�蟃邀 �>��>\ 宼Υ=.T摹 �觖S; Z鄜�!ㄠ傏�銹��9gi槾咰ぺ�!�# B鰻,欒;匵=刍,I�2UW�9$l╧嗒捋=Aj挄�;顊朅79鍇s*Y摈�;浱爺[嘙C撣�県f华]o栫{oY=1k�yV骋V惺隐5E8鏥k+譁扑蚛8疴计0X4D)�!!��?*|f縱� u洒《"鴛A谸T_矋��帬q矁6�4)k诂u襐�7�顃�'尛%;嬁蟟膦9s�9箈懫�,熵趲-45x鰀娐�8?�菢蟙�/Y|t��&LIL饾J`琛& �-G硉�/��PK! 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